Jatoi (Urdu: جتوئ‎), is the capital city of Jatoi Tehsil (administrative subdivision), in Muzaffargarh district, Punjab province, Pakistan. It is the 91st largest city of Pakistan by population.

The city has a Govt. Girls’ degree college. It is built recently in 2013. It has Govt. Boys Degree College & High Schools for Boys & Girls. It also has a large number of private institutions. There is Sardar Kauray Khan Jatoi park and the library is also going to built soon by Kauray Khan’s trust in the name of Sardar Kauray Khan Jatoi. Jatoi is an agricultural city, with very fertile soil and water provided by a canal system and tube wells, producing two or three crops in a year. Major crops are cotton, wheat, sugarcane, rice, and sunflower. The major fruits are mango, dates, and Jamun. Politicians include Sardar Abdul Qayyum Jatoi, Syed Abdullah Shah Bukhari and their sons, and later ones including Khan Muhammad Khan, Sami Ullah Khan Laghari, Syed Jameel Shah Bukhari. The city has a mosque, located in the Kotla Rehm Ali shah, a rural part of Jatoi Tehsil. This mosque is single means of spiritual as well as enjoying a place for people of Jatoi and concerning areas. The literacy rate is about 5%. The environment has been damaged by intensive felling of trees, but in 2012 more than 1000 trees were planted. About 10 KM away from Jatoi City there is the famous Mosque Jamia Sakeena-Tu-Sughra is situated.